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maxima sybr green/rox qpr master mix (2x)  (Thermo Fisher)


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    Structured Review

    Thermo Fisher maxima sybr green/rox qpr master mix (2x)
    Maxima Sybr Green/Rox Qpr Master Mix (2x), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/maxima+sybr+green+master+mix+(2x/maxima+syber+green+rox+master+mix+2x/pmc10620768__EMBJ___42___e113975___s012-929-36-43
    Average 90 stars, based on 1 article reviews
    maxima sybr green/rox qpr master mix (2x) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: A real-time qPCR assay to quantify Fusarium graminearum biomass in wheat kernels.
    Article Snippet: Head blight of wheat, caused by Fusarium graminearum sensu lato Schwabe [teleomorph Gibberella zeae (Schwein.). Petch] in the United States, and by Fusarium culmorum, Fusarium sporotrichioides and Fusarium avenaceum in Europe and Asia (Mesterházy et al. 2005), substantially decreases yields and contaminates grain with trichothecene mycotoxins.. In the United States, deoxynivalenol (DON) is the trichothecene toxin of greatest concern in wheat contaminated by F. graminearum (Chandler et al. 2003), whereas nivalenol is of greatest concern in other regions of the world (Placinta et al. 1999).

    Article Title: Repeated administration of a subanesthetic dose of ketamine results in impaired motor and cognitive behavior and differential expression of hippocampal P2X1 and P2X7 receptors in adult mice.
    Article Snippet: Ketamine hydrochloride serves multiple purposes, including its use as a general anesthetic, treatment for depression, and recreational drug.. In studies involving rodents, ketamine is utilized as a model for schizophrenia.. However, it is unclear whether age affects the behavioral response induced by repeated ketamine administration and if it modifies the expression levels of N-methyl-D-aspartate (NMDA) and α-amino-3-hydroxy-5-methyl-4isoxazolepropionic acid (AMPA) and purinergic receptors (P2X1, P2X4, P2X7).

    Sterility:

    Article Title: A real-time qPCR assay to quantify Fusarium graminearum biomass in wheat kernels.
    Article Snippet: Head blight of wheat, caused by Fusarium graminearum sensu lato Schwabe [teleomorph Gibberella zeae (Schwein.). Petch] in the United States, and by Fusarium culmorum, Fusarium sporotrichioides and Fusarium avenaceum in Europe and Asia (Mesterházy et al. 2005), substantially decreases yields and contaminates grain with trichothecene mycotoxins.. In the United States, deoxynivalenol (DON) is the trichothecene toxin of greatest concern in wheat contaminated by F. graminearum (Chandler et al. 2003), whereas nivalenol is of greatest concern in other regions of the world (Placinta et al. 1999).

    Concentration Assay:

    Article Title: A real-time qPCR assay to quantify Fusarium graminearum biomass in wheat kernels.
    Article Snippet: Head blight of wheat, caused by Fusarium graminearum sensu lato Schwabe [teleomorph Gibberella zeae (Schwein.). Petch] in the United States, and by Fusarium culmorum, Fusarium sporotrichioides and Fusarium avenaceum in Europe and Asia (Mesterházy et al. 2005), substantially decreases yields and contaminates grain with trichothecene mycotoxins.. In the United States, deoxynivalenol (DON) is the trichothecene toxin of greatest concern in wheat contaminated by F. graminearum (Chandler et al. 2003), whereas nivalenol is of greatest concern in other regions of the world (Placinta et al. 1999).

    Real-time Polymerase Chain Reaction:

    Article Title: A real-time qPCR assay to quantify Fusarium graminearum biomass in wheat kernels.
    Article Snippet: Head blight of wheat, caused by Fusarium graminearum sensu lato Schwabe [teleomorph Gibberella zeae (Schwein.). Petch] in the United States, and by Fusarium culmorum, Fusarium sporotrichioides and Fusarium avenaceum in Europe and Asia (Mesterházy et al. 2005), substantially decreases yields and contaminates grain with trichothecene mycotoxins.. In the United States, deoxynivalenol (DON) is the trichothecene toxin of greatest concern in wheat contaminated by F. graminearum (Chandler et al. 2003), whereas nivalenol is of greatest concern in other regions of the world (Placinta et al. 1999).

    Article Title: Immunomodulatory activity and control of Salmonella Enteritidis colonization in the intestinal tract of chickens by Lactobacillus based probiotic.
    Article Snippet: Lactobacillus-based probiotics (LBP) are used as competitive exclusion to control pathogenic enterobacterial infections and improve the weight gain in broiler chickens.. This study assessed the inhibition of Salmonella Enteritidis (SE) infection in one-week-old broiler chicks, using an experimental LBP containing four Lactobacillus strains isolated from chickens (L. acidophilus, L. fermentum, L. reuteri, L. salivarius).. The immunomodulatory effects of this treatment were evaluated, through the analysis of cytokines and influx of macrophages, , CD4+ and CD8+ T cells in the gut.

    SYBR Green Assay:

    Article Title: A real-time qPCR assay to quantify Fusarium graminearum biomass in wheat kernels.
    Article Snippet: Head blight of wheat, caused by Fusarium graminearum sensu lato Schwabe [teleomorph Gibberella zeae (Schwein.). Petch] in the United States, and by Fusarium culmorum, Fusarium sporotrichioides and Fusarium avenaceum in Europe and Asia (Mesterházy et al. 2005), substantially decreases yields and contaminates grain with trichothecene mycotoxins.. In the United States, deoxynivalenol (DON) is the trichothecene toxin of greatest concern in wheat contaminated by F. graminearum (Chandler et al. 2003), whereas nivalenol is of greatest concern in other regions of the world (Placinta et al. 1999).

    Article Title: Survival of Igα-Deficient Mature B Cells Requires BAFF-R Function.
    Article Snippet: .. Quantitative RT-PCR (qRT-PCR) was performed using Maxima SYBR Green Master Mix 2X (Thermo Scientific) in a 7500 Fast real-time system light cycler (Applied Biosystems) at an annealing temperature of 60 ̊C for 40 cycles. ..

    Article Title: Environmental Controls on Soil Microbial Communities in a Seasonally Dry Tropical Forest
    Article Snippet: .. Reactions were performed in a final volume of 20 μL of reaction mixture 492 containing 10 μl of Maxima SYBR Green Master Mix 2x (Thermo Scientific, MA), 1 μl of 493 DNA, 0.3 μM of each primer and a supplement of bovine serum albumin (BSA) or T4 Gene 32 494 Protein to both increase template detection sensitivity and suppress humic acid inhibition. ..

    Article Title: Immunomodulatory activity and control of Salmonella Enteritidis colonization in the intestinal tract of chickens by Lactobacillus based probiotic.
    Article Snippet: Lactobacillus-based probiotics (LBP) are used as competitive exclusion to control pathogenic enterobacterial infections and improve the weight gain in broiler chickens.. This study assessed the inhibition of Salmonella Enteritidis (SE) infection in one-week-old broiler chicks, using an experimental LBP containing four Lactobacillus strains isolated from chickens (L. acidophilus, L. fermentum, L. reuteri, L. salivarius).. The immunomodulatory effects of this treatment were evaluated, through the analysis of cytokines and influx of macrophages, , CD4+ and CD8+ T cells in the gut.

    Article Title: Molecular marker identification, antioxidant, antinociceptive, and anti-inflammatory responsiveness of malonic acid capped silver nanoparticle
    Article Snippet: .. RT-PCR was performed by using Maxima SYBR Green Master Mix 2X (Thermo Scientific) and their manufacturer’s manual was followed. .. The amplification of the samples was performed in PCR QuantStudio 7 Flex Thermalcycler (Thermo Scientific).

    Article Title: Repeated administration of a subanesthetic dose of ketamine results in impaired motor and cognitive behavior and differential expression of hippocampal P2X1 and P2X7 receptors in adult mice.
    Article Snippet: Ketamine hydrochloride serves multiple purposes, including its use as a general anesthetic, treatment for depression, and recreational drug.. In studies involving rodents, ketamine is utilized as a model for schizophrenia.. However, it is unclear whether age affects the behavioral response induced by repeated ketamine administration and if it modifies the expression levels of N-methyl-D-aspartate (NMDA) and α-amino-3-hydroxy-5-methyl-4isoxazolepropionic acid (AMPA) and purinergic receptors (P2X1, P2X4, P2X7).

    Quantitative RT-PCR:

    Article Title: Survival of Igα-Deficient Mature B Cells Requires BAFF-R Function.
    Article Snippet: .. Quantitative RT-PCR (qRT-PCR) was performed using Maxima SYBR Green Master Mix 2X (Thermo Scientific) in a 7500 Fast real-time system light cycler (Applied Biosystems) at an annealing temperature of 60 ̊C for 40 cycles. ..

    Inhibition:

    Article Title: Environmental Controls on Soil Microbial Communities in a Seasonally Dry Tropical Forest
    Article Snippet: .. Reactions were performed in a final volume of 20 μL of reaction mixture 492 containing 10 μl of Maxima SYBR Green Master Mix 2x (Thermo Scientific, MA), 1 μl of 493 DNA, 0.3 μM of each primer and a supplement of bovine serum albumin (BSA) or T4 Gene 32 494 Protein to both increase template detection sensitivity and suppress humic acid inhibition. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Molecular marker identification, antioxidant, antinociceptive, and anti-inflammatory responsiveness of malonic acid capped silver nanoparticle
    Article Snippet: .. RT-PCR was performed by using Maxima SYBR Green Master Mix 2X (Thermo Scientific) and their manufacturer’s manual was followed. .. The amplification of the samples was performed in PCR QuantStudio 7 Flex Thermalcycler (Thermo Scientific).



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    hV2R genetic and protein expression assessment in engineered CHO cell lines. A: Quantification results of AVPR2 -directed <t>RT-qPCR</t> performed on the two AVPR2 -expressing CHO cell lines compared to reference tissue samples. Results are shown as fold changes between the expression in the investigated samples and the expression in the positive reference, healthy human total kidney. B: Scanning confocal microscopy observation of CHO-304, CHO-3013 and the V2R-negative control CHO-K1 cell lines after Cy5-MQ232 staining. Up: Total staining using 100 nM Cy5-MQ232. Down: Non-specific staining using 100 nM Cy5-MQ232 in presence of 30 µM MQ232. Blue: DAPI (nuclear staining, 10 ms exposure at 352 nm, light gathered at 465 nm). Red: Cy5 (800 ms exposure at 650 nm, light gathered at 673 nm). C: Flow cytometry results obtained on freshly dissociated CHO-304, CHO-3013 and CHO-K1 cells stained using 100 nM Cy5-MQ232 (total signal, red curve) or 100 nM Cy5-MQ232 in presence of 30 µM of MQ232 (non-specific signal, blue curve). Light gathered at 680 nm, 30,000 events per acquisition, two acquisitions per independent experiment, n = 3). D: Specific V2R-linked fluorescence signal obtained through the subtraction of the mean fluorescence intensity after non-specific labeling from the mean fluorescence intensity after total labeling.
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    Image Search Results


    hV2R genetic and protein expression assessment in engineered CHO cell lines. A: Quantification results of AVPR2 -directed RT-qPCR performed on the two AVPR2 -expressing CHO cell lines compared to reference tissue samples. Results are shown as fold changes between the expression in the investigated samples and the expression in the positive reference, healthy human total kidney. B: Scanning confocal microscopy observation of CHO-304, CHO-3013 and the V2R-negative control CHO-K1 cell lines after Cy5-MQ232 staining. Up: Total staining using 100 nM Cy5-MQ232. Down: Non-specific staining using 100 nM Cy5-MQ232 in presence of 30 µM MQ232. Blue: DAPI (nuclear staining, 10 ms exposure at 352 nm, light gathered at 465 nm). Red: Cy5 (800 ms exposure at 650 nm, light gathered at 673 nm). C: Flow cytometry results obtained on freshly dissociated CHO-304, CHO-3013 and CHO-K1 cells stained using 100 nM Cy5-MQ232 (total signal, red curve) or 100 nM Cy5-MQ232 in presence of 30 µM of MQ232 (non-specific signal, blue curve). Light gathered at 680 nm, 30,000 events per acquisition, two acquisitions per independent experiment, n = 3). D: Specific V2R-linked fluorescence signal obtained through the subtraction of the mean fluorescence intensity after non-specific labeling from the mean fluorescence intensity after total labeling.

    Journal: Theranostics

    Article Title: Specific in vivo detection of V2R-positive metastatic ccRCC using a toxin-based PET radioligand

    doi: 10.7150/thno.126311

    Figure Lengend Snippet: hV2R genetic and protein expression assessment in engineered CHO cell lines. A: Quantification results of AVPR2 -directed RT-qPCR performed on the two AVPR2 -expressing CHO cell lines compared to reference tissue samples. Results are shown as fold changes between the expression in the investigated samples and the expression in the positive reference, healthy human total kidney. B: Scanning confocal microscopy observation of CHO-304, CHO-3013 and the V2R-negative control CHO-K1 cell lines after Cy5-MQ232 staining. Up: Total staining using 100 nM Cy5-MQ232. Down: Non-specific staining using 100 nM Cy5-MQ232 in presence of 30 µM MQ232. Blue: DAPI (nuclear staining, 10 ms exposure at 352 nm, light gathered at 465 nm). Red: Cy5 (800 ms exposure at 650 nm, light gathered at 673 nm). C: Flow cytometry results obtained on freshly dissociated CHO-304, CHO-3013 and CHO-K1 cells stained using 100 nM Cy5-MQ232 (total signal, red curve) or 100 nM Cy5-MQ232 in presence of 30 µM of MQ232 (non-specific signal, blue curve). Light gathered at 680 nm, 30,000 events per acquisition, two acquisitions per independent experiment, n = 3). D: Specific V2R-linked fluorescence signal obtained through the subtraction of the mean fluorescence intensity after non-specific labeling from the mean fluorescence intensity after total labeling.

    Article Snippet: The reaction volume was then diluted to a concentration of 10 ng/μL and the qPCR reaction carried out on Hard-Shell High Profile 96 well plates (Bio-Rad, USA) using 5 μL of the complimentary DNA (cDNA) corresponding to 50 ng total RNA, both primers at 500 nM and 2X Maxima SYBR Green/Fluorescein qPCR Master Mix (Thermo Fisher Scientific, USA) according to the manufacturer's recommendations.

    Techniques: Expressing, Quantitative RT-PCR, Confocal Microscopy, Negative Control, Staining, Flow Cytometry, Fluorescence, Labeling

    In vivo visualization of V2R + tumors in mccRCC representative mice models. A: Representative composite images taken at 4 h after the i.v. injection of 20 nmol/kg for the [ 18 F]F-MQ232 in CHO-304 xenografted NMRI-Foxn1 nu/nu mice (6.8 ± 1.1 MBq, 274 ± 43 MBq/kg), CHO-3013 xenografted NMRI-Foxn1 nu/nu mice (3.8 ± 1.2 MBq, 150 ± 47 MBq/kg), Caki-1 xenografted NMRI-Foxn1 nu/nu mice (3.8 ± 1.2 MBq, 152 ± 47 MBq/kg) and Renca grafted BALB/cJ mice (2.5 ± 0.9 MBq, 99 ± 37 MBq/kg). Images shown are the result of a fusion between the Computed Tomography (CT) image (grey scale) and the PET image (false colors). B: Summary table of the main PET imaging indicators obtained after 4 hours of the i.v. injection of 20 nmol/kg for the [ 18 F]F-MQ232 in CHO-304 xenografted NMRI-Foxn1 nu/nu mice (6.8 ± 1.1 MBq, 274 ± 43 MBq/kg), CHO-3013 xenografted NMRI-Foxn1 nu/nu mice (3.8 ± 1.2 MBq, 150 ± 47 MBq/kg), Caki-1 xenografted NMRI-Foxn1 nu/nu mice (3.8 ± 1.2 MBq, 152 ± 47 MBq/kg) and Renca grafted BALB/cJ mice (2.5 ± 0.9 MBq, 99 ± 37 MBq/kg). C: Flow cytometry results obtained on freshly dissociated Caki-1 and Renca cells stained using 100 nM Cy5-MQ232 (total signal, red curve) or 100 nM Cy5-MQ232 in presence of 30 µM of MQ232 (non-specific signal, blue curve). Light gathered at 680 nm, 30,000 events per acquisition, two acquisitions per independent experiment, n = 3). D: Quantification results of AVPR2 -directed RT-qPCR performed on Caki-1 and Renca cells and tumors resulting from their implantation. Results are shown as fold changes between the expression in the investigated samples and the expression in the positive reference, healthy human total kidney. E: Quantification results of AVPR2 -directed RT-qPCR performed on 8 ccRCC and mccRCC biopsies (P1 to P8). Three samples came from low grade primary tumors (grade ≤ 2) (P1 to P3, in green), two from high grade primary tumors (grade 3 or 4) (P4 and P5, in blue), and three from distant metastases (P6 to P8, in purple). Results are shown as fold changes between the expression in the investigated samples and the expression in the positive reference, healthy human total kidney.

    Journal: Theranostics

    Article Title: Specific in vivo detection of V2R-positive metastatic ccRCC using a toxin-based PET radioligand

    doi: 10.7150/thno.126311

    Figure Lengend Snippet: In vivo visualization of V2R + tumors in mccRCC representative mice models. A: Representative composite images taken at 4 h after the i.v. injection of 20 nmol/kg for the [ 18 F]F-MQ232 in CHO-304 xenografted NMRI-Foxn1 nu/nu mice (6.8 ± 1.1 MBq, 274 ± 43 MBq/kg), CHO-3013 xenografted NMRI-Foxn1 nu/nu mice (3.8 ± 1.2 MBq, 150 ± 47 MBq/kg), Caki-1 xenografted NMRI-Foxn1 nu/nu mice (3.8 ± 1.2 MBq, 152 ± 47 MBq/kg) and Renca grafted BALB/cJ mice (2.5 ± 0.9 MBq, 99 ± 37 MBq/kg). Images shown are the result of a fusion between the Computed Tomography (CT) image (grey scale) and the PET image (false colors). B: Summary table of the main PET imaging indicators obtained after 4 hours of the i.v. injection of 20 nmol/kg for the [ 18 F]F-MQ232 in CHO-304 xenografted NMRI-Foxn1 nu/nu mice (6.8 ± 1.1 MBq, 274 ± 43 MBq/kg), CHO-3013 xenografted NMRI-Foxn1 nu/nu mice (3.8 ± 1.2 MBq, 150 ± 47 MBq/kg), Caki-1 xenografted NMRI-Foxn1 nu/nu mice (3.8 ± 1.2 MBq, 152 ± 47 MBq/kg) and Renca grafted BALB/cJ mice (2.5 ± 0.9 MBq, 99 ± 37 MBq/kg). C: Flow cytometry results obtained on freshly dissociated Caki-1 and Renca cells stained using 100 nM Cy5-MQ232 (total signal, red curve) or 100 nM Cy5-MQ232 in presence of 30 µM of MQ232 (non-specific signal, blue curve). Light gathered at 680 nm, 30,000 events per acquisition, two acquisitions per independent experiment, n = 3). D: Quantification results of AVPR2 -directed RT-qPCR performed on Caki-1 and Renca cells and tumors resulting from their implantation. Results are shown as fold changes between the expression in the investigated samples and the expression in the positive reference, healthy human total kidney. E: Quantification results of AVPR2 -directed RT-qPCR performed on 8 ccRCC and mccRCC biopsies (P1 to P8). Three samples came from low grade primary tumors (grade ≤ 2) (P1 to P3, in green), two from high grade primary tumors (grade 3 or 4) (P4 and P5, in blue), and three from distant metastases (P6 to P8, in purple). Results are shown as fold changes between the expression in the investigated samples and the expression in the positive reference, healthy human total kidney.

    Article Snippet: The reaction volume was then diluted to a concentration of 10 ng/μL and the qPCR reaction carried out on Hard-Shell High Profile 96 well plates (Bio-Rad, USA) using 5 μL of the complimentary DNA (cDNA) corresponding to 50 ng total RNA, both primers at 500 nM and 2X Maxima SYBR Green/Fluorescein qPCR Master Mix (Thermo Fisher Scientific, USA) according to the manufacturer's recommendations.

    Techniques: In Vivo, Injection, Computed Tomography, Imaging, Flow Cytometry, Staining, Quantitative RT-PCR, Expressing